Journal: Bioengineering

Article Title: Comparison of Bioengineered Scaffolds for the Induction of Osteochondrogenic Differentiation of Human Adipose-Derived Stem Cells

doi: 10.3390/bioengineering11090920

Figure Lengend Snippet: hASC survival and differentiation in GelMA hydrogels. ( A ) CCK8 assay of hASCs encapsulated in the GelMA hydrogel at different time points (1 week intervals). Results are reported as mean ± SEM of n = 3 samples/group. ( B ) Representative confocal imaging of Dead/live fluorescence staining of hASCs in the GelMA hydrogel after 3 weeks of culture. Red cells are dead while green cells are alive. ( C ) Alcian blue staining of GelMA hydrogel after 24 h and 3 weeks of culture. ( D ) Alizarin red staining of GelMA hydrogel after 24 h and 3 weeks of culture. ( E , F ) Confocal imaging of immunofluorescence for COL2A1 (green) and OCN (red) in hASCs encapsulated in GelMA hydrogel after 3 weeks of culture.

Article Snippet: Briefly, samples were incubated with rabbit polyclonal anti-collagen type II alpha 1 (COL2A1) antibody (Merck) and rabbit polyclonal anti-osteocalcin (OCN) antibody (Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: CCK-8 Assay, Imaging, Fluorescence, Staining, Immunofluorescence

Journal: Bioengineering

Article Title: Comparison of Bioengineered Scaffolds for the Induction of Osteochondrogenic Differentiation of Human Adipose-Derived Stem Cells

doi: 10.3390/bioengineering11090920

Figure Lengend Snippet: hASC survival and differentiation in PEGDA scaffold. ( A ) CCK8 assay of hASCs seeded in the PEGDA scaffold at different time points (1 week intervals). Results are reported as mean ± SEM of n = 3 samples/group. T test: * p < 0.05. ( B ) Representative confocal imaging of Dead/live fluorescence staining of hASCs seeded in the PEGDA scaffold after 3 weeks of culture. ( C ) Confocal imaging of immunofluorescence for COL2A1 (green) and OCN (red) in hASCs seeded in the PEGDA scaffold after 3 weeks of culture.

Article Snippet: Briefly, samples were incubated with rabbit polyclonal anti-collagen type II alpha 1 (COL2A1) antibody (Merck) and rabbit polyclonal anti-osteocalcin (OCN) antibody (Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: CCK-8 Assay, Imaging, Fluorescence, Staining, Immunofluorescence

Journal: Bioengineering

Article Title: Comparison of Bioengineered Scaffolds for the Induction of Osteochondrogenic Differentiation of Human Adipose-Derived Stem Cells

doi: 10.3390/bioengineering11090920

Figure Lengend Snippet: hASC survival and differentiation in celery-based scaffold. ( A ) SEM imaging of a single hASC inside a niche of the celery-based scaffold. ( B ) CCK8 assay of hASCs seeded in the celery-based scaffold at different time points (1 week intervals). Results are reported as mean ± SEM of n = 3 samples/group. T test: * p < 0.05. ( C ) Representative confocal imaging of Dead/live fluorescence staining of hASCs seeded in the scaffold after 3 weeks of culture. ( D ) Confocal 3D stack and ( E ) the projection of hASC distribution inside the scaffold. ( F ) Confocal imaging of immunofluorescence for COL2A1 (green) and OCN (red) in hASCs seeded in the PEGDA scaffold after 3 weeks of culture.

Article Snippet: Briefly, samples were incubated with rabbit polyclonal anti-collagen type II alpha 1 (COL2A1) antibody (Merck) and rabbit polyclonal anti-osteocalcin (OCN) antibody (Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: Imaging, CCK-8 Assay, Fluorescence, Staining, Immunofluorescence

Journal: Frontiers in Pharmacology

Article Title: Intermittent Fasting Inhibits High-Fat Diet–Induced Atherosclerosis by Ameliorating Hypercholesterolemia and Reducing Monocyte Chemoattraction

doi: 10.3389/fphar.2021.719750

Figure Lengend Snippet: Sequences of primers for qRT-PCR.

Article Snippet: Mouse anti–smooth muscle α-actin (SMA) monoclonal antibody and rabbit anti-collagen type III alpha 1 (COL3A1) polyclonal antibody were purchased from Affinity Biosciences (Cincinnati, OH, United States).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Intermittent Fasting Inhibits High-Fat Diet–Induced Atherosclerosis by Ameliorating Hypercholesterolemia and Reducing Monocyte Chemoattraction

doi: 10.3389/fphar.2021.719750

Figure Lengend Snippet: Intermittent fasting increases collagen content via inducing COL1A1 and COL3A1 expression. (A,B) The aortic root cross sections were used to determine collagen content by Picrosirius red staining with quantitative analysis of collagen positive areas. * p < 0.05 ( n = 6). (C,D) The expression of COL1A1, COL3A1, MMP2, and MMP9 in plaques were determined by immunofluorescent staining with the quantification of positive staining areas. * p < 0.05, ns: not significant ( n = 6). E : LDLR−/− mice were divided into the control or IF group ( n = 6/group). After 2 weeks HFD feeding ad libitum or IF treatment, the mice aorta samples were collected. The expression of COL1A1, COL3A1, MMP2, and MMP9 were determined by qRT-PCR. * p < 0.05, ns: not significant ( n = 6).

Article Snippet: Mouse anti–smooth muscle α-actin (SMA) monoclonal antibody and rabbit anti-collagen type III alpha 1 (COL3A1) polyclonal antibody were purchased from Affinity Biosciences (Cincinnati, OH, United States).

Techniques: Expressing, Staining, Control, Quantitative RT-PCR